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Award Information
Proposal Number: 0511198
Proposal Title: Channel to Droplet Sample Extraction and Purification Using Electrowetting Device
Topic Number: H-SB05.1-001
Phase: Phase I
Topic Title: ADVANCED SAMPLE PROCESSING OF LIQUID, OR SOLID OR AEROSOL SAMPLES, OR A COMBINATION OF TWO OR THREE
Organization: Core MicroSolutions Inc.
Address: 1100 Glendon Avenue 17th Floor
Los Angeles, CA 90024-3588  
Abstract: To develop advanced "channel-to-droplet" sample extraction and purification functions on a compact cartridge that is free of sensitive and complex microfluidic components, Core Microsolutions (CMSS) and Professor Sung Cho of the University of Pittsburgh propose to integrate Electrowetting-on-Dielectric (EWOD) droplet handling and in-droplet concentration capabilties with well proven channel-based separation methods to: a) separate target particles, b) extract the concentrated target particles in a mobile droplet, c) bifurcate the particle mix within the droplet using Dielectrophoretic separation methods for a 2nd level of concentration effects, and then d) drive the target-rich droplet to a sensing point or pipette extraction site. This proposed DHS work will develop the sample extraction and purification methods needed to complement CMSS' ongoing two year $1M NIH Biodefense Phase I grant that will produce the hardware and driving systems needed for novel bacteria immuno-capture and transduction on an EWOD sensing cartridge. This hybridization of channel and droplet-based sample handling on a generic slide cartridge will enable elegant transfer of channel-based separation products to an array of electronic, optical, surface plasmon resonance (SPR), mass spectrometry (MS) instrumentation. Comparisons in cost/performance will be made against existing methods at the end of Phase I.
Award/Contract Number: NBCHC050123
Period of Performance: 06/01/2005 - 12/15/2005
Award/Contract Value: $99,929.00
Award/Obligated Amount: $99,929.00