Topic Information Award/Contract Number Proposal Information Company Performance

Preparation of Environmental Samples for Biological Detection

D12PC00287 FY11.1-H-SB011.1-002-0009-II
(FY11.1 Phase II)
A Novel Fractionation and Concentration System for Improved Biological Detection

InnovaPrep LLC
132 east main street
Drexel, MO 64742-0068


Detection of bioaerosols is a cornerstone in the protection of our nation from bioterrorist and pandemic events. Rapid bioaerosol detection systems have advanced dramatically in recent years, but the lack of integratable automated sample preparation for these systems remains a major deficiency. Three significant issues are (a) a lack of an interface between large environmental samples and sample preparation and detection components requiring very small volumes, (b) deficiencies in removing inhibitory environmental material and (c) an inability to separate, and thus, differentiate whole pathogens from exogenous materials derived from those pathogens. InnovaPrep is developing a novel membrane-based fractionation and concentration system capable of removing inhibitors and separating whole pathogens and exogenous materials into two highly-concentrated volumes. The proposed system will be exceedingly rapid; with the capability to fractionate, prepare, and concentrate 10 milliliter environmental aerosol samples (without sample splitting) into 100 microliter volumes in less than 15 minutes, and deliver the samples ready for identification. The system will be composed of updatable snap-together modules that can be operated as-needed for different systems, providing separately concentrated nucleic acids and proteins from free solution and from whole cells . A portion of the viable whole cells will also be archived by the system. All disposable components in the modules will have operational lifetimes of up to hundreds of samples, thereby allowing for straightforward integration into autonomous biodetection systems. The system being developed here is applicable to multibillion dollar commercial and government markets that are growing at a rate of 18.3 percent.

Assess Ability to use Eye Tracking and Pupil Dilation to Determine Intent to Deceive

HSHQDC09C00115 0812003
(FY08.1 Phase II)
Assess Ability to use Eye Tracking and Pupil Dialtion to Determine Intent to Deceive - Phase II

Bio-Behavior Analysis Systems, LLC
STE 307
St. Louis, MO 63117-1639


We propose to run 3 experiments to refine and utilize measures of oculomotor activity and pupil diameter change to assess an individual`s malicious or deceptive intent. We also propose to concurrently develop the software and determine the hardware necessary for online, real-time assessment of malintent based on prior research results and the results of the proposed experiments. In addition to oculomotor and pupil activity, we plan on collecting other physiological measures (e.g. electrodermal activity, respiration, cardiovascular measures) with the intent of developing a broader base of measures allowing for assessment of malicious or deceptive intent. Experiments will be conducted within the constraints imposed by the DHS Future Attribute Screening Technology (FAST) environment. The focus is on measures that can be recorded unobtrusively (without the attachment of sensors) and that can provide immediate feedback.