|Proposal Title:||Aerosol Collection into Low Analysis Volumes|
|Topic Title:||AEROSOL COLLECTION INTO LOW ANALYSIS VOLUMES (ACLAV)|
|Organization:||Aerosol Dynamics Inc.|
|Address:||935 Grayson St.
Berkeley, CA 94710-2640
|Abstract:||Detection systems for aeropathogens require the concentration of airborne particles from large volumes of air into small volumes of liquid. This proposal addresses this need through droplet encapsulation of airborne particles, with subsequent capture into a microliter-sized collection reservoir. Droplet encapsulation is accomplished through our company's proven, patented growth tube technology whereby a region of water vapor supersaturation is created in a thermally diffusive, laminar flow. No steam or temperature extremes are required. Flow lines are smooth, facilitating the transport of coarse particles. It provides efficient capture of particles in the small-submicrometer size range, as is needed for virus detection. The collection step is gentle, and non-drying, to yield a concentrated yet viable sample. A 100L/min collection system will be constructed to provide collection into a 300 microliter liquid volume. The size-dependent collection efficiency, internal losses and efficiency of elution will be measured over the size range from 0.2 to 10 micrometre using inert liquid and solid particles. The final prototype system will be tested with bare and agglomerated bacterial spores with assessment of collection efficiency, viability, and suitability for PCR analyses.|
|Period of Performance:||08/06/2007 - 02/05/2011|