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Award Information
Proposal Number: 0512016
Proposal Title: A Novel Electrokinetics-Driven, Integrated Microfluidic Cartridge for Sample Preparation from Complex Matrices
Topic Number: H-SB05.1-001
Phase: Phase II
Topic Title: ADVANCED SAMPLE PROCESSING OF LIQUID, OR SOLID OR AEROSOL SAMPLES, OR A COMBINATION OF TWO OR THREE
Organization: CFD Research Corporation
Address: 215 Wynn Dr.
5th Floor
Huntsville, AL 35805-1926  
Abstract: Sample preparation has been recognized as the single most important challenge to be faced in the development of detect-to-warn (DTW) systems (NRC, 2005). Available commercial sample preparation technologies are expensive, slow (30 min to 2 hours) and require trained laboratory technicians for operation. Addressing this need, we propose to design, fabricate, demonstrate, and validate a novel integrated microfluidic cartridge exploiting advanced electrokinetics for preparation of complex liquid samples for genomic analysis. The Phase I study successfully demonstrated three critical elements: (1) sorting of bacterial cells (2) electric field driven microbial lysis, and (3) DNA capture and elution in a microfluidic extraction chamber. Physical prototypes of the components were fabricated and experimentally demonstrated. During Phase II, these components will be optimized and integrated along with a novel pre-concentrator to develop an integrated microfluidics sample preparation card. The integrated cartridge will be validated in challenge situations mixing simulants and threat agents with real-world contaminants. Proprietary design tools will be used to guide component development and layout optimization. An experienced, multi-disciplinary team with expertise in microfluidics engineering, microfabrication, and agent testing has been assembled.
Award/Contract Number: D06PC75222 (formerly NBCHC060070)
Period of Performance: 04/24/2006 - 10/31/2008
Award/Contract Value: $749,966.00
Award/Obligated Amount: $749,966.00